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    Sample Preparation

    Phosphopeptide Stage-tip


    Phosphorylation is among the most widespread post-translational modifications in nature, and it has been estimated that more than 30% of the proteins in a given mammalian cell at some point during their expression are phosphorylated. Phosphorylation and dephosphorylation of proteins regulates a large number of biological processes such as signal transduction, molecular recognition and interaction, and other cellular events. Phosphoproteome mapping can be challenging due to low abundance, dynamic and much less ionization efficiency.

    The Preciex® Phosphopeptide enrichment Stage-tip uses a TiO2 Resin with optimized protocol and buffers resulting in a higher yield of phosphopeptides ready for direct MS analysis without the need for additional clean up. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures.

     

    Process of use: centrifugation, vacuum, positive pressure.

    Formats: 10µL and 200µL.

    For any other offline or online format, contact us.

     

     

    *200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.

     

     

    Features of Phosphopeptide Stage-tip:

    • Higher selectivity for multiply Phosphorylate peptides
    • Achieve ready-to-measure Phosphopeptides

    • Suitable for manual and automated processing

    Common product Specifications:

    Sample

    Phosphopeptide

    Chromatographic bed

    TiO2 resin

    Volume

    200μL

    Capacity

    4ug

    pH Range

    1-14

    Temperature

    4-40°C

    Quantity

    96 Tips

    Detection Method

    HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy


    Ordering Information:

    Description

    Format

    Capacity

    Part Number

    Preciex Phosphopeptide

    10μL

    1μg

    2108011

    Preciex Phosphopeptide

    200μL

    4μg

    2108042

    Preciex Phosphopeptide

    200μL

    15μg

    2108153

     

    User Guide

    http://guantengmaoyi.com/public/upload/202207/20220728214952.pdf

    Material Safety Data Sheets (MSDS)


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